Regulatory

Part:BBa_K4303021:Design

Designed by: Darsh Vithlani   Group: iGEM22_ICT-Mumbai   (2022-10-11)


5'UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We first BioBricked it according to the RFC10 standards then added the RBS BBa0030 downstream and the promoter BBaJ23100 upstream. This 5'UTR was tested in a monomeric RFP BBaE1010.


Source

This is a mutated version of cspF which is BBa_K4303017. The position nine has a mutation of T to A.

References